Determination of Acetyl Salicylic Acid in Aspirin tablets

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Lana Mohammed Ali Mariwan Abdulla Hama Salih Omed Ismael Hayder


Drug analysis is an important method for determination of active components of any therapeutic drugs. HPLC, Spectrometry, Direct titration and Back-titration are common effective techniques in drug analysis. They were used in this study in a comparative way for the determination of the correct amount of acetyl salicylic acid in aspirin formulations. The aim of this study is to analysis of aspirin and compares both Direct titration and Back-titration techniques to the standard HPLC technique in order to find alternative, the easy and cheaper procedure to assess the quality of active components of medications aspirin in particular in Sulaimani city. Additionally, Spectrophotometric technique were also used and compared with the standard HPLC method. The samples were collected from different local drug stores in which distribute medications to pharmacies and they were from different companies. The analyses were performed after preparing a solution from each of the samples with a standard solutions for titration procedures. Both direct and back-titration techniques were used to find the concentration of active components in aspirin samples then compared with the data from standard HPLC as a reference value. The study showed that those companies which provide medications to this region were used a standard amount of acetyl salicylic acid in aspirin tablets. The result from Spectrophotometric method showed more accurate than both direct and indirect titration when compared to the reference value from standard HPLC technique due to the high rate of accuracy and precision of this method. However, the result also showed some inconsistent data in terms of the back titration compared to direct titration due to the inaccuracy in back titration. This study concluded with the necessity for quality control to use all these techniques for monitoring all medications which interring this region.


Drug analysis, HPLC, Titration, Back-Titration, Aspirin, Spectrophotometer, Acetyl Salicylic Acid.


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[1] B. Nikolin, B. Imamović, S. Medanhodžić-Vuk, and M. Sober, "High performance liquid chromatography in pharmaceutical analyses," Bosnian Journal of Basic Medical Sciences, vol. 4, 2004.
[2] K. Schrör, Acetylsalicylic Acid. Germany: British Library Cataloguing-in-Publication Data, 2009.
[3] P. Silverman, M. Seskar, D. Kanter, P. Schweizer, J. P. Metraux, and I. Raskin, "Salicylic Acid in Rice Biosynthesis, Conjugation and Possible Role," Plant Physiology, vol. 108, pp. 633-639, 1995.
[4] Bayer Inc. (2014, Product Monograph. Available:
[5] M. C. Koester, "An overview of the physiology and pharmacology of Aspirin and Nonsteroidal Anti-inflammatory Drugs," Journal of Atheletic Training, vol. 28, pp. 252-259, 1993.
[6] C. S. Kwok and Y. K. Loke, "Critical overview on the Benefits and Harms of Aspirin," Pharmaceuticals, vol. 3, pp. 1491-1506, 2010.
[7] M. Yusuf, et al., "Salicylic Acid: Physiological Roles in Plants," ed, 2013, pp. 15-30.
[8] D. C. Harris, Quantitative Chemical Analysis, 7 ed., 2007.
[9] G. Motan and A. Pui, "Studies of different types of aspirin by spectrophotometric methods," ACTA CHEMICA IASI, vol. 22, pp. 155-164, 2014.
[10] G. Murtaza, et al., "Development of a UV-spectrophotometric method for the simultaneous determination of aspirin and paracetamol in tablets," Scientific Research and Essays vol. 6, 2011.
[11] A. M. El-Didamony, M. Z. Saad, and N. O. Saleem, "Spectrophotometric determination of some analgesic drugs in pharmaceutical formulations using N-bromosuccinimide as an oxidant," Journal of the Association of Arab Universities for Basic and Applied Sciences, vol. 17, pp. 43-50, 2015/04/01/ 2015.
[12] S. S. Mohamed, "Comparative Analytical Study for Determination of Acetyl salicylic Acid in Bulk and Pharmaceutical Formulations," Journal of Al-Nahrain University, vol. 16, pp. 1-10, 2013.
[13] M. H. Bhuyian, P. D. H. Rashid, A. Islam, and M. Tareque, "Development and Validation of Method for Determination of Clobetasol Propionate and Salicylic Acid from Pharmaceutical Dosage form by HPLC," British journal of Pharmaceutical Research, vol. 7, pp. 375-385, 2015.
[14] J. Fernández, M. Castro, and M. Valcárcel, "Automatic continuous on-line monitoring of salicylic acid and acetylsalicylic acid in pharmaceuticals," Journal of Automatic Chemistry, vol. 12, pp. 263-266, 1990.
[15] V. Martínez-Merino. (2007, Determination of the free Salicylic Acid concentration in Aspirin by forming Fe+3 complexes Available:
[16] B. Scientific, "Fluorimetric Determination of Acetylsalicylic Acid in Aspirin," British Pharmacopeia, vol. 2, 1993.
[17] A. Sani, A. Ahmed, T. Alemika, M. A. Sani, A. Ojuolape, A. Sani, A. Bukola, S. Zakama, and I. Mohammed, "Comparative quantitaive analysis of different brands of 300 mg Aspirin tablet marketed in Maiduguri Metropolitan Council (MMC)," International Journal of Pharma Sciences and Researc, vol. 3, 2012.
[18] R. T. Suhas Gurav, Vishali Salunkhe, Devprakash, Senthilkumar G.P., "Spectrophotometric Determination of Clopidogrel Bisulfate in Pharmaceutical formulations," American Journal of Pharmtech Research, vol. 1, 2011.
[19] S. Mitic, G. Miletic, A. Pavlović, S. Tošić, and S. Sunaric, "Quantitative Analysis of Acetylsalicylic Acid in Commercial Pharmaceutical Formulations and Human Control Serum Using Kinetic Spectrophotometr," Acta Chim. Slov. , vol. 55, pp. 508-515, 2008.
[20] Bellevue College. (2017, Titration of synthesised Aspirin. Available:
[21] C. Cofan and C. Radovan, "Anodic Determination of Acetyl salicylic Acid at a Mildly Oxidized Boron-Doped Diamond Electrode in Sodium Sulphate Medium," International Journal of Electrochemistry, 2011.