Influence of Light Intensity on Tobacco Responses to Drought Stress

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Brwa Rasool


The influence of high irradiance, drought stress and their cross-talk were explored in tobacco plants (Nicotiana tobaccum) grown under low light (250 μmol m-2 s-1) irradiance (LL) and high (1600 μmol m-2 s-1) irradiance (HL) then exposed to water deficient condition for 7 or 14 days. The detached leaves of HL-treated plants showed less water loss compared to LL plants. The HL-treated and 7 days drought-stressed plants had higher fresh and dry weights, as well as water content than the LL and drought-stressed leaves. The survival rate in 21 days drought-stressed plants after 3 days of re-watering was 50% in HL-grown and 0% in LL-grown plants. 

A transcriptome profiling analysis of the tobacco responses to light intensity highlights the increased abundance of a large group of drought-related transcripts including DROUGHT-RESPONSIVE ELEMENT BINDING FACTORS (DREBs), C-REPEAT/DROUGHT-RESPONSIVE BINDING FACTOR 1 (CBF1), GLYCINE-RICH RNA BINDING PROTEINS (GRPs), WRKY33 and MYCs transcription factors, as well as zeaxanthin epoxidase, which play as a regulator of plant responses to water deficient condition.

These findings identify light-dependent changes in the cell redox state that limit water loss and enhance plant responses to drought stress.


High light stress, drought stress, light memory, cross-tolerance, redox regulation


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[1] W. Wang, B. Vinocur, and A. Altman, "Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance," (in eng), Planta, vol. 218, no. 1, pp. 1-14, Nov 2003, doi: 10.1007/s00425-003-1105-5.
[2] S. Wani, V. Kumar, V. Shriram, and S. Sah, "Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants," The Crop Journal, vol. 4, p. 162, 04/01 2016, doi: 10.1016/j.cj.2016.01.010.
[3] Y. Osakabe, K. Osakabe, K. Shinozaki, and L. S. Tran, "Response of plants to water stress," (in eng), Front Plant Sci, vol. 5, p. 86, 2014, doi: 10.3389/fpls.2014.00086.
[4] N. Suzuki, S. Koussevitzky, R. Mittler, and G. Miller, "ROS and redox signalling in the response of plants to abiotic stress," (in eng), Plant Cell Environ, vol. 35, no. 2, pp. 259-70, Feb 2012, doi: 10.1111/j.1365-3040.2011.02336.x.
[5] D. Golldack, C. Li, H. Mohan, and N. Probst, "Tolerance to drought and salt stress in plants: Unraveling the signaling networks," (in eng), Front Plant Sci, vol. 5, p. 151, 2014, doi: 10.3389/fpls.2014.00151.
[6] M. Hussain et al., "Drought stress in sunflower: Physiological effects and its management through breeding and agronomic alternatives," Agricultural Water Management, vol. 201, pp. 152-166, 03/31 2018, doi: 10.1016/j.agwat.2018.01.028.
[7] D. Bartels and R. Sunkar, "Drought and Salt Tolerance in Plants," Critical Reviews in Plant Sciences, vol. 24, no. 1, pp. 23-58, 2005/02/23 2005, doi: 10.1080/07352680590910410.
[8] J. A. Chowdhury, M. Karim, Q. Khaliq, A. Ahmed, and M. Shawquat, "Effect of drought stress on gas exchange characteristics of four soybean genotypes," Bangladesh Journal of Agricultural Research, vol. 41, p. 195, 06/16 2016, doi: 10.3329/bjar.v41i2.28215.
[9] Y. Jiang and B. Huang, "Drought and Heat Stress Injury to Two Cool-Season Turfgrasses in Relation to Antioxidant Metabolism and Lipid Peroxidation," Crop Science - CROP SCI, vol. 41, 03/01 2001, doi: 10.2135/cropsci2001.412436x.
[10] A. S. Moffat, "Plant genetics. Finding new ways to protect drought-stricken plants," (in eng), Science, vol. 296, no. 5571, pp. 1226-9, May 17 2002, doi: 10.1126/science.296.5571.1226.
[11] G. M. Pastori and C. H. Foyer, "Common components, networks, and pathways of cross-tolerance to stress. The central role of "redox" and abscisic acid-mediated controls," (in eng), Plant Physiol, vol. 129, no. 2, pp. 460-8, Jun 2002, doi: 10.1104/pp.011021.
[12] R. Mittler, "Abiotic stress, the field environment and stress combination," (in eng), Trends Plant Sci, vol. 11, no. 1, pp. 15-9, Jan 2006, doi: 10.1016/j.tplants.2005.11.002.
[13] R. M. Bostock, "Signal crosstalk and induced resistance: straddling the line between cost and benefit," (in eng), Annu Rev Phytopathol, vol. 43, pp. 545-80, 2005, doi: 10.1146/annurev.phyto.41.052002.095505.
[14] J. C. Cushman and H. J. Bohnert, "Genomic approaches to plant stress tolerance," (in eng), Curr Opin Plant Biol, vol. 3, no. 2, pp. 117-24, Apr 2000, doi: 10.1016/s1369-5266(99)00052-7.
[15] M. Fujita et al., "Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks," (in eng), Curr Opin Plant Biol, vol. 9, no. 4, pp. 436-42, Aug 2006, doi: 10.1016/j.pbi.2006.05.014.
[16] S. Karpiński, M. Szechyńska-Hebda, W. Wituszyńska, and P. Burdiak, "Light acclimation, retrograde signalling, cell death and immune defences in plants," (in eng), Plant Cell Environ, vol. 36, no. 4, pp. 736-44, Apr 2013, doi: 10.1111/pce.12018.
[17] C. H. Foyer and G. Noctor, "Redox regulation in photosynthetic organisms: signaling, acclimation, and practical implications," (in eng), Antioxid Redox Signal, vol. 11, no. 4, pp. 861-905, Apr 2009, doi: 10.1089/ars.2008.2177.
[18] S. Karpinski, H. Gabrys, A. Mateo, B. Karpinska, and P. M. Mullineaux, "Light perception in plant disease defence signalling," (in eng), Curr Opin Plant Biol, vol. 6, no. 4, pp. 390-6, Aug 2003, doi: 10.1016/s1369-5266(03)00061-x.
[19] M. Sierla, M. Rahikainen, J. Salojärvi, J. Kangasjärvi, and S. Kangasjärvi, "Apoplastic and chloroplastic redox signaling networks in plant stress responses," (in eng), Antioxid Redox Signal, vol. 18, no. 16, pp. 2220-39, Jun 1 2013, doi: 10.1089/ars.2012.5016.
[20] M. Sierla, C. Waszczak, T. Vahisalu, and J. Kangasjärvi, "Reactive Oxygen Species in the Regulation of Stomatal Movements," (in eng), Plant Physiol, vol. 171, no. 3, pp. 1569-80, Jul 2016, doi: 10.1104/pp.16.00328.
[21] S. Karpinski and M. Szechyńska-Hebda, "Cellular light memory, photo-electrochemical and Redox retrograde signaling in plants," Journal of Biotechnology, Computational Biology and Bionanotechnology, vol. 9.73, pp. 27-39, 01/01 2012, doi: 10.5114/bta.2012.46566.
[22] P. Mühlenbock et al., "Chloroplast signaling and LESION SIMULATING DISEASE1 regulate crosstalk between light acclimation and immunity in Arabidopsis," (in eng), Plant Cell, vol. 20, no. 9, pp. 2339-56, Sep 2008, doi: 10.1105/tpc.108.059618.
[23] A. Fini et al., "Drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in Fraxinus ornus leaves: an excess light stress affair?," (in eng), J Plant Physiol, vol. 169, no. 10, pp. 929-39, Jul 1 2012, doi: 10.1016/j.jplph.2012.02.014.
[24] H. Lichtenthaler and C. Buschmann, "Chlorophylls and carotenoids: Measurements and characterization by UV-Vis spectroscopy," Food Analytical Chemistry: Pigments, Colorants, Flavors, Texture and Bioactive Food Components, pp. 171-178, 01/01 2005.
[25] B. Karpinska et al., "The redox state of the apoplast influences the acclimation of photosynthesis and leaf metabolism to changing irradiance," (in eng), Plant Cell Environ, vol. 41, no. 5, pp. 1083-1097, May 2018, doi: 10.1111/pce.12960.
[26] Q. Liu, N. Zhao, K. Yamaguch-Shinozaki, and K. Shinozaki, "Regulatory role of DREB transcription factors in plant drought, salt and cold tolerance," Chinese Science Bulletin, vol. 45, pp. 970-975, 06/01 2000, doi: 10.1007/BF02884972.
[27] N. Liu et al., "Cloning and functional characterization of PpDBF1 gene encoding a DRE-binding transcription factor from Physcomitrella patens," (in eng), Planta, vol. 226, no. 4, pp. 827-38, Sep 2007, doi: 10.1007/s00425-007-0529-8.
[28] T. H. Hsieh, J. T. Lee, Y. Y. Charng, and M. T. Chan, "Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficient stress," (in eng), Plant Physiol, vol. 130, no. 2, pp. 618-26, Oct 2002, doi: 10.1104/pp.006783.
[29] T. H. Hsieh et al., "Heterology expression of the Arabidopsis C-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato," (in eng), Plant Physiol, vol. 129, no. 3, pp. 1086-94, Jul 2002, doi: 10.1104/pp.003442.
[30] J. S. Kim et al., "Glycine-rich RNA-binding protein 7 affects abiotic stress responses by regulating stomata opening and closing in Arabidopsis thaliana," (in eng), Plant J, vol. 55, no. 3, pp. 455-66, Aug 2008, doi: 10.1111/j.1365-313X.2008.03518.x.
[31] M. Czolpinska and M. Rurek, "Plant Glycine-Rich Proteins in Stress Response: An Emerging, Still Prospective Story," (in eng), Front Plant Sci, vol. 9, p. 302, 2018, doi: 10.3389/fpls.2018.00302.
[32] X. Wang, B. Du, M. Liu, N. Sun, and X. Qi, "Arabidopsis Transcription Factor WRKY33 Is Involved in Drought by Directly Regulating the Expression of CesA8," American Journal of Plant Sciences, vol. 04, pp. 21-27, 01/01 2013, doi: 10.4236/ajps.2013.46A004.
[33] A. Banerjee and A. Roychoudhury, "WRKY proteins: signaling and regulation of expression during abiotic stress responses," (in eng), ScientificWorldJournal, vol. 2015, p. 807560, 2015, doi: 10.1155/2015/807560.
[34] T. Javed, R. Shabbir, A. Ali, I. Afzal, U. Zaheer, and S. J. Gao, "Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement," (in eng), Plants (Basel), vol. 9, no. 4, Apr 10 2020, doi: 10.3390/plants9040491.
[35] N. Schwarz et al., "Tissue-specific accumulation and regulation of zeaxanthin epoxidase in Arabidopsis reflect the multiple functions of the enzyme in plastids," (in eng), Plant Cell Physiol, vol. 56, no. 2, pp. 346-57, Feb 2015, doi: 10.1093/pcp/pcu167.
[36] M. W. Bianchi, C. Roux, and N. Vartanian, "Drought regulation of GST8, encoding the Arabidopsis homologue of ParC/Nt107 glutathione transferase/peroxidase," (in eng), Physiol Plant, vol. 116, no. 1, pp. 96-105, Sep 2002, doi: 10.1034/j.1399-3054.2002.1160112.x.
[37] J. Xu et al., "Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress," (in eng), PLoS One, vol. 10, no. 9, p. e0136960, 2015, doi: 10.1371/journal.pone.0136960.
[38] M. Szechyńska-Hebda, J. Kruk, M. Górecka, B. Karpińska, and S. Karpiński, "Evidence for light wavelength-specific photoelectrophysiological signaling and memory of excess light episodes in Arabidopsis," (in eng), Plant Cell, vol. 22, no. 7, pp. 2201-18, Jul 2010, doi: 10.1105/tpc.109.069302.
[39] G. Zervoudakis, G. Salachas, Kaspiris, and Konstantopoulou, "Influence of Light Intensity on Growth and Physiological Characteristics of Common Sage (Salvia officinalis L.)," Brazilian Archives of Biology and Technology, vol. 55, pp. 89-95, 02/01 2012, doi: 10.1590/S1516-89132012000100011.
[40] J. F. d. C. Gonçalves, D. C. d. S. Barreto, U. M. d. Santos Junior, A. V. Fernandes, P. d. T. B. Sampaio, and M. S. Buckeridge, "Growth, photosynthesis and stress indicators in young rosewood plants (Aniba rosaeodora Ducke) under different light intensities," Brazilian Journal of Plant Physiology, vol. 17, pp. 325-334, 2005. [Online]. Available:
[41] M. Mielke and B. Schaffer, "Photosynthetic and growth responses of Eugenia uniflora L. seedlings to soil flooding and light intensity," Environmental and Experimental Botany, vol. 68, pp. 113-121, 05/06 2010, doi: 10.1016/j.envexpbot.2009.11.007.
[42] X.-y. Yang, X.-f. Ye, G.-s. Liu, H.-q. Wei, and Y. Wang, "Effects of light intensity on morphological and physiological characteristics of tobacco seedlings," Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban, vol. 18, pp. 2642-5, 12/01 2007.
[43] K. Müller et al., "A red light-controlled synthetic gene expression switch for plant systems," (in eng), Mol Biosyst, vol. 10, no. 7, pp. 1679-88, Jul 2014, doi: 10.1039/c3mb70579j.
[44] X.-y. Yao, X. Liu, Z.-g. Xu, and X.-l. Jiao, "Effects of light intensity on leaf microstructure and growth of rape seedlings cultivated under a combination of red and blue LEDs," Journal of Integrative Agriculture, vol. 16, pp. 97-105, 01/31 2017, doi: 10.1016/S2095-3119(16)61393-X.
[45] D. Fanourakis et al., "Stomatal behavior following mid- or long-term exposure to high relative air humidity: A review," (in eng), Plant Physiol Biochem, vol. 153, pp. 92-105, 2020/08// 2020, doi: 10.1016/j.plaphy.2020.05.024.
[46] H. An and Z. P. Shangguan, "Effects of light intensity and nitrogen application on the growth and photosynthetic characteristics of Trifolium repens L," Shengtai Xuebao/ Acta Ecologica Sinica, vol. 29, pp. 6017-6024, 01/01 2009.
[47] Y. Wang, Q. Guo, and M. Jin, "[Effects of light intensity on growth and photosynthetic characteristics of Chrysanthemum morifolium]," (in chi), Zhongguo Zhong Yao Za Zhi, vol. 34, no. 13, pp. 1632-5, Jul 2009.
[48] R. Mittler, S. Vanderauwera, M. Gollery, and F. Van Breusegem, "Reactive oxygen gene network of plants," (in eng), Trends Plant Sci, vol. 9, no. 10, pp. 490-8, Oct 2004, doi: 10.1016/j.tplants.2004.08.009.
[49] M. A. Torres and J. L. Dangl, "Functions of the respiratory burst oxidase in biotic interactions, abiotic stress and development," (in eng), Curr Opin Plant Biol, vol. 8, no. 4, pp. 397-403, Aug 2005, doi: 10.1016/j.pbi.2005.05.014.
[50] K. J. Dietz, I. Turkan, and A. Krieger-Liszkay, "Redox- and Reactive Oxygen Species-Dependent Signaling into and out of the Photosynthesizing Chloroplast," (in eng), Plant Physiol, vol. 171, no. 3, pp. 1541-50, Jul 2016, doi: 10.1104/pp.16.00375.
[51] H. Yu et al., "Activated expression of an Arabidopsis HD-START protein confers drought tolerance with improved root system and reduced stomatal density," (in eng), Plant Cell, vol. 20, no. 4, pp. 1134-51, Apr 2008, doi: 10.1105/tpc.108.058263.
[52] L. Yu et al., "Arabidopsis enhanced drought tolerance1/HOMEODOMAIN GLABROUS11 confers drought tolerance in transgenic rice without yield penalty," (in eng), Plant Physiol, vol. 162, no. 3, pp. 1378-91, Jul 2013, doi: 10.1104/pp.113.217596.
[53] Z. Zhu et al., "Overexpression of AtEDT1/HDG11 in Chinese Kale (Brassica oleracea var. alboglabra) Enhances Drought and Osmotic Stress Tolerance," (in eng), Front Plant Sci, vol. 7, p. 1285, 2016, doi: 10.3389/fpls.2016.01285.
[54] M. Chaves, J. Flexas, and C. Pinheiro, "Photosynthesis Under Drought and Salt Stress: Regulation Mechanisms From Whole Plant to Cell," Annals of botany, vol. 103, pp. 551-60, 08/01 2008, doi: 10.1093/aob/mcn125.
[55] Y. G. Shen, W. K. Zhang, S. J. He, J. S. Zhang, Q. Liu, and S. Y. Chen, "An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress," (in eng), Theor Appl Genet, vol. 106, no. 5, pp. 923-30, Mar 2003, doi: 10.1007/s00122-002-1131-x.
[56] Y. Wang et al., "Drought tolerance evaluation of tobacco plants transformed with different set of genes under laboratory and field conditions," Science Bulletin, vol. 60, pp. 616-628, 03/01 2015, doi: 10.1007/s11434-015-0748-5.
[57] R. F. Ahmed, M. Irfan, H. A. Shakir, M. Khan, and L. Chen, "Engineering drought tolerance in plants by modification of transcription and signalling factors," Biotechnology & Biotechnological Equipment, vol. 34, no. 1, pp. 781-789, 2020/01/01 2020, doi: 10.1080/13102818.2020.1805359.
[58] K. Datta, N. Baisakh, M. Ganguly, S. Krishnan, K. Yamaguchi Shinozaki, and S. K. Datta, "Overexpression of Arabidopsis and rice stress genes' inducible transcription factor confers drought and salinity tolerance to rice," (in eng), Plant Biotechnol J, vol. 10, no. 5, pp. 579-86, Jun 2012, doi: 10.1111/j.1467-7652.2012.00688.x.
[59] P. Bhatnagar-Mathur et al., "Transgenic peanut overexpressing the DREB1A transcription factor has higher yields under drought stress," Molecular Breeding, 09/19 2013, doi: 10.1007/s11032-013-9952-7.
[60] R. S. Wang et al., "Common and unique elements of the ABA-regulated transcriptome of Arabidopsis guard cells," (in eng), BMC Genomics, vol. 12, p. 216, May 9 2011, doi: 10.1186/1471-2164-12-216.
[61] D. Singh and A. Laxmi, "Transcriptional regulation of drought response: a tortuous network of transcriptional factors," (in eng), Front Plant Sci, vol. 6, p. 895, 2015, doi: 10.3389/fpls.2015.00895.
[62] Y. Yoon, D. Seo, H. Shin, H. Kim, C. Kim, and G. Jang, "The Role of Stress-Responsive Transcription Factors in Modulating Abiotic Stress Tolerance in Plants," Agronomy, vol. 10, p. 788, 06/01 2020, doi: 10.3390/agronomy10060788.
[63] K. Shinozaki and K. Yamaguchi-Shinozaki, "Gene networks involved in drought stress response and tolerance," (in eng), J Exp Bot, vol. 58, no. 2, pp. 221-7, 2007, doi: 10.1093/jxb/erl164.
[64] M. Boter, O. Ruíz-Rivero, A. Abdeen, and S. Prat, "Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis," (in eng), Genes Dev, vol. 18, no. 13, pp. 1577-91, Jul 1 2004, doi: 10.1101/gad.297704.
[65] H. Y. Park et al., "Overexpression of Arabidopsis ZEP enhances tolerance to osmotic stress," (in eng), Biochem Biophys Res Commun, vol. 375, no. 1, pp. 80-5, Oct 10 2008, doi: 10.1016/j.bbrc.2008.07.128.
[66] Z. Zhang et al., "MsZEP, a novel zeaxanthin epoxidase gene from alfalfa (Medicago sativa), confers drought and salt tolerance in transgenic tobacco," (in eng), Plant Cell Rep, vol. 35, no. 2, pp. 439-53, Feb 2016, doi: 10.1007/s00299-015-1895-5.